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| Bowtie2 v2.4.1 | 02/28/20 |
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Please cite: Langmead B, Salzberg S. Fast gapped-read alignment with Bowtie 2. Nature Methods. 2012, 9:357-359. |
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Links
Related Tools
| Bowtie: Ultrafast short read alignment |
| Crossbow: Genotyping, cloud computing |
| Myrna: Cloud, differential gene expression |
| Tophat: RNA-Seq splice junction mapper |
| Cufflinks: Isoform assembly, quantitation |
| Lighter: Fast error correction |
Indexes
| H. sapiens, NCBI GRCh38 | 3.5 GB |
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H. sapiens, NCBI GRCh38 with 1KGenomes major SNPs |
3.5 GB |
| How we built this, FASTA | |
| H. sapiens, UCSC hg19 | 3.5 GB |
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H. sapiens, UCSC hg19 with 1KGenomes major SNPs |
3.5 GB |
| How we built this, FASTA | |
| M. musculus, UCSC mm10 | 3.2 GB |
| M. musculus, UCSC mm9 | 3.2 GB |
| R. norvegicus, UCSC rn4 | 3.1 GB |
Related publications
- Langmead B, Wilks C., Antonescu V., Charles R. Scaling read aligners to hundreds of threads on general-purpose processors. Bioinformatics. bty648.
- Langmead B, Salzberg S. Fast gapped-read alignment with Bowtie 2. Nature Methods. 2012, 9:357-359.
- Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biology 10:R25.
Contributors
- Ben Langmead
- Daehwan Kim
- Rone Charles
- Chris Wilks
- Valentin Antonescu
Related links
Tools that use Bowtie (or Bowtie 2):
- mRNA sequencing:
- Myrna (paper)
- TopHat, TopHat 2 (paper)
- Cufflinks (paper)
- Trinity (paper)
- MapSplice (paper)
- SpliceSeq (paper)
- RNASEQR (paper)
- RSEM (paper)
- GENE-counter (paper)
- RobiNA (paper)
- ExpressionPlot (paper)
- RUM (paper)
- BowStrap (paper)
- ContextMap (paper)
- eXpress (paper)
- ReXpress (paper)
- TrueSight (paper)
- PASTA (paper)
- QRNAseq
- Bisulfite-seq:
- BSmooth (paper)
- Bismark (paper)
- MethylCoder (paper)
- BS-Seeker (paper)
- B-SOLANA (paper)
- Bicycle
- Bison
- Re-seqeuncing:
- Assembly and scaffolding:
- Workflow:
- Galaxy (paper)
- Chipster (paper)
- GAPSS
- Unipro UGENE (paper)
- Biopieces
- InSilico DB (paper)
- LONI Pipeline (paper)
- KBase
- bioKepler
- Structural variants:
- Gene fusion:
- TopHat Fusion (paper)
- deFuse (paper)
- Comrad (paper)
- ShortFuse (paper)
- FusionHunter (paper)
- SnowShoes-FD (paper)
- FusionFinder (paper)
- Chimerascan (paper)
- FusionQ (paper)
- Metagenomics:
- Small RNA:
- isomiRID (paper)
- Deqor v3 (paper)
- NEXT-RNAi (design of genome-wide RNAi libraries)
- miREvo (paper)
- miRanalyzer (paper)
- MapMi (paper)
- shortran (paper)
- miRDeep2 (paper)
- YM500 (paper)
- starBase (paper)
- Other:
- PADUA (alignment of DNA to protein databases; paper)
- SeqTrimMap (sequential trimming and mapping; paper)
- mPrime (primer design)
- EMIGRE (ribosomal genes reconstruction; paper)
- TAPDANCE (identifies CISs in transposon junction fragment insertion data; paper)
- Paparazzi (reconstruction of viral genomes from small RNA reads; paper)
- CaPSID (pathogen detection; paper)
- EuPathDB (eukaryotic pathogen detection; paper)
OS distributions with Bowtie packages
Commercial products using Bowtie:
Published protocols using Bowtie 1 and/or 2:
- Wang S, et al. Target analysis by integration of transcriptome and ChIP-seq data with BETA. Nature Protocols.
- Hisano M, et al. Genome-wide chromatin analysis in mature mouse and human spermatozoa. Nature Protocols.
- Becker AH, et al. Selective ribosome profiling as a tool for studying the interaction of chaperones and targeting factors with nascent polypeptide chains and ribosomes. Nature Protocols.
- Anders S, et al. Count-based differential expression analysis of RNA sequencing data using R and Bioconductor. Nature Protocols.
- Wan Y, et al. Genome-wide mapping of RNA structure using nuclease digestion and high-throughput sequencing. Nature Protocols.
- Haas BJ, et al. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nature Protocols.
- Ingolia NT, et al. Genome-Wide Annotation and Quantitation of Translation by Ribosome Profiling. Current Protocols in Molecular Biology.
- Baslan T, et al. Genome-wide copy number analysis of single cells. Nature Protocols.
- Trapnell C, et al. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nature Protocols.
- Ingolia NT, et al. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nature Protocols.
- Bardet AF, et al. A computational pipeline for comparative ChIP-seq analyses. Nature Protocols.
- Simon JS, et al. Using formaldehyde-assisted isolation of regulatory elements (FAIRE) to isolate active regulatory DNA. Nature Protocols.
- Goodman AL, et al. Identifying microbial fitness determinants by insertion sequencing using genome-wide transposon mutant libraries. Nature Protocols.
- Fanelli M, et al. Chromatin immunoprecipitation and high-throughput sequencing from paraffin-embedded pathology tissue. Nature Protocols.
- Christodoulou DC, et al.Quantification of Gene Transcripts with Deep Sequencing Analysis of Gene Expression (DSAGE) Using 1 to 2 micrograms Total RNA. Current Protocols in Molecular Biology.
