Microsatellites, also known as simple sequence repeats (SSRs), are repetitive DNA typically consist of 1-6 nucleotides. These repeats are found in almost all organisms genome including chloroplast and mitochondria. Microsatellites are widely used molecular markers and play important role in many areas of biological research.
To compare microsatellites between species; we have developed an automated pipeline named AutomAted RepeaT Identifier (AARTI). This pipeline identifies and categorize microsatellites as common (found in more than one organism), polymorphic (showing length variation between organisms), and unique (found only in an organism) among nucleotide sequences.
AARTI initially uses MIcroSAtellite identification tool (MISA; a PERL script) to mine microsatellites in nucleotide sequences and further apply Basic Local Alignment Search Tool (BLAST) to categorize identified microsatellites as common, polymorphic, and unique considering homologous relationship between sequences.
User can submit a file of nucleotide sequences in FASTA format (with .fasta/ .fna/ .fa extension) for execution and receive a link of the results page through an email (if provided) after completion of the job.